Selected Publications

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By Héctor A. Becerril, Randall M. Stoltenberg, Dean R. Wheeler, Robert C. Davis, John N. Harb, and Adam T. Woolley
Abstract: Three-branched DNA molecules have been designed and assembled from oligonucleotide components. These nucleic acid constructs contain double- and single-stranded regions that control the hybridization behavior of the assembly. Specific localization of a single streptavidin molecule at the center of the DNA complex has been investigated as a model system for the directed placement of nanostructures. Highly selective silver and copper metallization of the DNA template has also been characterized. Specific hybridization of these DNA complexes to oligonucleotide-coupled nanostructures followed by metallization should provide a bottom-up self-assembly route for the fabrication and characterization of discrete three-terminal nanodevices.
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By Travis Hughes, Bradley Strongin, Viksita Vijayvergiya, David D. Busath, and Robert C. Davis (et al.)
Abstract: We report the observation of influenza A M2 (M2) incorporated in a dipalmitoylphosphatidylcholine (DPPC) supported planar bilayer on mica, formed by use of a modified vesicle fusion method from proteoliposomes and visualized with contact mode atomic force microscopy. Incubation of proteoliposomes in a hyperosmotic solution and increased DPPC/M2 weight ratios improved supported planar bilayer formation by M2/DPPC proteoliposomes. M2's extra-bilayer domains were observed as particles estimated to protrude 1-1.5 nm above the bilayer surface and <4 nm in diameter. Particle density was 5-18% of the nominal tetramer density. Movement of observable M2 particles was independent of the probe tip. The mean lateral diffusion coefficient (D) of M2 was 4.4 &PLUSMN; 1.0 x 10(-14) cm(2)/s. Eighty-two percent of observable particles were mobile on the observable timescale (D) > 6 x 10(-15) cm(2)/s). Protein-protein interactions were also observed directly.
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By Travis Hughes, Bradley Strongin, David D. Busath, and Robert C. Davis (et al.)
Abstract:
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By Jorj I. Owen, Travis L. Niederhauser, Brent A. Wacaser, Marc P. Christenson, Robert C. Davis, and Matthew R. Linford
Abstract: This paper describes three advances in lab on a chip technology. First, it is shown that chemomechanical surface patterning can be performed using a commercially available liquid handler that has undergone only minor modifications. These capabilities are demonstrated by making and then characterizing smaller hydrophobic corrals, made with a diamond tip, than have previously been reported. Hydrophobic corrals are small enclosures on a surface that are ringed by hydrophobic lines. They hold droplets of high surface tension solutions. They allow a surface to be subdivided into individually addressable elements, thus providing a platform for conducting many simultaneous surface experiments with small (down to ca. 1 muL) liquid volumes. An important consequence of this work is that it makes chemomechanical surface patterning, which is a valuable and straightforward method for surface modification, much more accessible to the technical community. Second, it is shown that an entire array of hydrophobic corrals can be simultaneously coated with polyelectrolyte multilayers, but that the hydrophobic corrals still retain the ability to hold liquids after this deposition. The robotic arm of the liquid handler is again employed to manufacture this ultrathin film. Finally, as a demonstration of the capability of this technology to create complex patterned arrays on surfaces from solution for biological or nanostructured materials applications, and again employing the liquid handler, polyelectrolyte-coated hydrophobic corrals are individually addressed and loaded with a solution containing gold nanoparticles for independently specified times. The density and morphology of deposited nanoparticle monolayers were studied by scanning electron microscopy. The deposition of gold nanoparticles onto a chip occurred at a constant rate (0.5% min(-1)) over the range of times studied.
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By Jed D. Whittaker, Ghaleb A. Husseini, Matthew R. Linford, and Robert C. Davis (et al.)
Abstract: A self-aligned thin-film deposition technique was developed to mechanically attach carbon nanotubes to surfaces for the fabrication of structurally robust nanotube-based nanomechanical devices. Single-walled carbon nanotubes were grown by thermal chemical-vapor deposition (CVD) across 150-nm-wide SiO2 trenches. The nanotubes were mechanically attached to the trench tops by selective silicon tetraacetate-based SiO2 CVD. No film was deposited on the nanotubes where they were suspended across the trenches. (C) 2003 American Institute of Physics.
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By Yit-Yian Lua, Travis L. Niederhauser, Brent A. Wacaser, Adam T. Woolley, Robert C. Davis, and Matthew R. Linford (et al.)
Abstract: We recently reported that monolayers on silicon are formed, and silicon surfaces concomitantly patterned, when native oxide-coated silicon is scribed with a diamond-tipped instrument in the presence of reactive liquids. Notably, monolayers were prepared (and are prepared in this work) in an open laboratory with reagents that are not degassed. However, while this method is facile, the features originally produced using 2-3 N of force on a diamond tip are irregular, broad (similar to100 mum), and deep (similar to5 mum). Reducing the force to 0.08 N using an improved tip holder yields narrower features (similar to10 mum), but the best features made with a diamond tip using the lighter force still remain quite deep (similar to0.1 mum) and rough. Here we show that substantially sharper and shallower features are produced by (a) Wetting hydrogen-terminated silicon with a reactive compound and (b) scribing it with a (1)/(32) in. tungsten carbide ball with a low force (similar to0.08 N). It is remarkable that W the depth of these features is only 10-20 Angstrom and (ii) their edge widths are sharp (submicron resolution). The resulting features are invisible to the naked eye but are observable by atomic force microscopy, scanning electron microscopy, and time-of-flight secondary ion mass spectrometry. Both Si(100) and Si(111) were successfully modified. Miniature hydrophobic corrals made with this technique were loaded with solutes, for example, colloidal carbon, semiconductor nanocrystals, and DNA, from aqueous solutions with a simple dip. Under appropriate conditions colloidal carbon selectively deposits onto functionalized lines but not in between them.